Based upon restriction fragment length polymorphism (RFLP) analysis using two restriction endonucleases (HaeIII and MboI) 77 of the 92 clones belonged to seven different clone families containing three or more clones, while 15 of the clones were unique.
Twelve clones representing each of the seven most abundant clone families as well as five additional unique clones were sequenced for identification.
BLAST (Basic Local Alignment Search Tool) searches [ Altschul et al.
Eleven of the clones were 97 to 99 similar to uncultured Group I marine Crenarchaeotes previously identified from various hydrothermal vent, subseafloor, and deep ocean trench habitats, while one of the clones was 95 similar to a Group II Euryarchaeon, clone SBAR1A (GenBank accession no.
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